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KMID : 0616120190500020148
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Korean Journal of Pharmacognosy
2019 Volume.50 No. 2 p.148 ~ p.153
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Isolation and Quantitative Analysis of Schisandrin, Gomisin A and Gomisin M2 From Schisandra chinensis
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Kim Young-Eun
Kim Eun-Nam
Jeong Gil-Saeng
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Abstract
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The lignan compound of Schisandra chinensis Baill. (Schizandraceae) has been reported to have a variety of biological activities such as liver protection, neuroprotection, anti-oxidant and anti-diabetes. In this study, we investigated a quantitative analytical method for schisandrin, gomisin A and gomisin M2 isolated from S. chinensis by high-performance liquid chromatography-ultraviolet spectroscopy (HPLC-UV). The isolated compounds were identified by the analysis of 1H- and 13C-NMR spectroscopic data. The results showed that calibration curves of three compounds indicated great linearity with a correlation coefficient (R2) of schisandrin 0.9983, gomisin A 0.9982 and gomisin M2 0.9986. The limits of detection (LOD) of schisandrin, gomisin A and gomisin M2 were 0.14, 0.07 and 0.05 μg/ml and the limits of quantification (LOQ) were 0.42, 0.22 and 0.14 μg/ml. Intra-day and inter-day precisions of schisandrin were 0.40~1.44%, 0.07~1.02% gomsin A were 0.22~0.52%, 0.10~0.63%, gomisin M2 were 0.40~0.99%, 0.81~2.88%. In result, contents of schisandrin, gomisin A and gomisin M2 in ethanol extract of S. chinensis were 25.95±0.15, 2.51±0.02 and 2.17±0.07 mg/g.
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KEYWORD
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HPLC, Quantitative determination, Schisandra chinensis, Gomisin M2, Gomisin A , Schisandrin
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